B Cell Receptor Signaling: Methods and Protocols

Front Matter ....Pages i-xi
 Activation-Induced Cytidine Deaminase Aided In Vitro Antibody Evolution  (Lili An, Chuan Chen, Ruiqi Luo, Yun Zhao, Haiying Hang)....Pages 1-14
 Analyzing Mouse B Cell Responses Specific to LCMV Infection  (Yaxing Hao, Zhirong Li, Yifei Wang, Xiaobing Liu, Lilin Ye)....Pages 15-38
 Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors  (Zhiyong Yang, Christopher D. C. Allen)....Pages 39-49
 Biophysical Techniques to Study B Cell Activation: Single-Molecule Imaging and Force Measurements  (Ivan Rey, David A. Garcia, Brittany A. Wheatley, Wenxia Song, Arpita Upadhyaya)....Pages 51-68
 DNA-Based Probes for Measuring Mechanical Forces in Cell-Cell Contacts: Application to B Cell Antigen Extraction from Immune Synapses  (Katelyn M. Spillane, Pavel Tolar)....Pages 69-80
 Deriving Quantitative Cell Biological Information from Dye-Dilution Lymphocyte Proliferation Experiments  (Koushik Roy, Maxim Nikolaievich Shokhirev, Simon Mitchell, Alexander Hoffmann)....Pages 81-94
 Flow Cytometry Analysis of mTOR Signaling in Antigen-Specific B Cells  (Qizhao Huang, Haoqiang Wang, Lifan Xu, Jianjun Hu, Pengcheng Wang, Yiding Li et al.)....Pages 95-109
 Ex Vivo Culture Assay to Measure Human Follicular Helper T (Tfh) Cell-Mediated Human B Cell Proliferation and Differentiation  (Xin Gao, Lin Lin, Di Yu)....Pages 111-119
 B Cell Receptor Signaling and Compartmentalization by Confocal Microscopy  (Anurag R. Mishra, Akanksha Chaturvedi)....Pages 121-129
 Imaging the Interactions Between B Cells and Antigen-Presenting Cells  (Jia C. Wang, Madison Bolger-Munro, Michael R. Gold)....Pages 131-161
 In Vivo Tracking of Particulate Antigen Localization and Recognition by B Lymphocytes at Lymph Nodes  (Yolanda R. Carrasco)....Pages 163-169
 Study B Cell Antigen Receptor Nano-Scale Organization by In Situ Fab Proximity Ligation Assay  (Kathrin Kläsener, Jianying Yang, Michael Reth)....Pages 171-181
 Single-Particle Tracking of Cell Surface Proteins  (Laabiah Wasim, Bebhinn Treanor)....Pages 183-192
 The Use of Intravital Two-Photon and Thick Section Confocal Imaging to Analyze B Lymphocyte Trafficking in Lymph Nodes and Spleen  (Chung Park, Il-Young Hwang, John H. Kehrl)....Pages 193-205
 Time-Lapse Förster Resonance Energy Transfer Imaging by Confocal Laser Scanning Microscopy for Analyzing Dynamic Molecular Interactions in the Plasma Membrane of B Cells  (Hae Won Sohn, Joseph Brzostowski)....Pages 207-224
 Understanding of B Cell Receptor Signaling Through a Photo-Activatable Antigen Presentation System  (Jing Wang, Zhengpeng Wan, Wanli Liu)....Pages 225-234
 Use of Streptolysin O-Induced Membrane Damage as a Method of Studying the Function of Lipid Rafts During B Cell Activation  (Heather Miller, Wenxia Song)....Pages 235-241
 Visualization and Quantitative Analysis of the Actin Cytoskeleton Upon B Cell Activation  (Vid Šuštar, Marika Vainio, Pieta K. Mattila)....Pages 243-257
Back Matter ....Pages 259-262